The fuel injection characteristics of Dimethyl Ether(DME) were calculated and compared with the calculated results of diesel fuel using a simulation model of an in-line diesel injection system in order to clarify the differences between the injection characteristics of the two fuels. Moreover, numerical analyses for the DME injection were performed while changing the fuel parameters and the injection system parameters in order to estimate the effects of these parameters on the fuel injection characteristics. The effects of some of these parameters were evaluated by experimental results conducted in a constant volume vessel. Furthermore, the spray tip penetration was calculated using the computed results of the injection pressure. As a result of this study, the injection characteristics of the DME fuel are basically confirmed. By the macroscopic analyses of these spray characteristics, the DME spray behavior in a combustion chamber can be estimated.