Repetitive movements have been found to affect assembly operations in many ways such as increasing the risk of injuries, increasing the cost of production, and reduction in the quality of products. This has been a big problem for industries. The method adopted by these studies seems to pose more injuries to workers as workers need to perform a task to the extreme level of pain to determine if repetitive injuries will occur or not. The method of modeling and simulation of human operations is a valid technique that is effective, but could be complex. Some of the modeling and simulation software packages make use of such guidelines as NIOSH, Snook and Ciriello, RULA, REBA, and Biomechanics single action analysis. However, various applications of these tools in actual ergonomic studies tend to be very time consuming and trivial due to the lack of a valid framework to guide the process. The objective of this paper is to present a detailed sequence of steps for injury analysis given an existing case study. T his framework can be used in conjunction with Delmia V5 software package to optimize various human operations given an existing assembly line.