This paper deals with experimental study of in-cylinder tumble flows in a single-cylinder, four-stroke, two-valve internal combustion engine using a pentroof-offset-bowl piston under non-reacting conditions with four intake manifold orientations at an engine speed of 1000 rev/min., during suction and compression strokes using particle image velocimetry. Two-dimensional in-cylinder tumble flow measurements and analysis are carried out in combustion space on a vertical plane passing through cylinder axis. Ensemble average velocity vectors are used to analyze the tumble flows. Tumble ratio (TR) and average turbulent kinetic energy (TKE) are evaluated and used to characterize the tumble flows. From analysis of results, it is found that at end of compression stroke, 90° intake manifold orientation shows an improvement in TR and TKE compared other intake manifold orientations considered. Also, 90° intake manifold orientation shows an improvement in TR and TKE are higher by about 2.8 and 1.2 times respectively compared to standard intake manifold orientation. The present study will be useful in understanding effect of intake manifold orientation on the in-cylinder tumble flows under real engine conditions..