An experimental study is performed to investigate the fuel impingement on cylinder walls and piston top inside a direct-injection spark-ignition engine with optical access to the cylinder. Three different fuels, namely, E85, E50 and gasoline are used in this work. E85 represents a blend of 85 percent ethanol and 15 percent gasoline by volume. Experiments are performed at different load conditions with the engine speeds of 1500 and 2000 rpm. Two types of fuel injectors are used; (i) High-pressure production injector with fuel pressures of 5 and 10 MPa, and (ii) Low-pressure production-intent injector with fuel pressure of 3 MPa. In addition, the effects of split injection are also presented and compared with the similar cases of single injection by maintaining the same amount of fuel for the stoichiometric condition. Novel image processing algorithms are developed to analyze the fuel impingement quantitatively on cylinder walls and piston top inside the engine cylinder. Qualitative details of spray tip penetration are also presented to reveal the effects of ethanol fuels compared to that of gasoline. It is found that the split injection is an effective way to reduce the overall fuel impingement on in-cylinder surfaces. No significant difference is observed on fuel spray pattern when gasoline is compared with E50 and E85. However, spray tip penetration is slightly higher with gasoline than that of ethanol fuels. Results also show that the wall impingement is higher with gasoline compared to ethanol fuels.