A 500 hours endurance test was performed with a heavy-duty engine (Euro IV); MAN type D 0836 LFL 51 equipped with a PM-Kat®. As fuel 100% biodiesel was used that met the European specification EN 14214. The 500 hours endurance test included both the European stationary and transient cycle (ESC and ETC) as well as longer stationary phases. During the test, regulated emissions (carbon monoxide, nitrogen oxides, hydrocarbons and particulate matter), the particle number distribution and the aldehydes emission were continuously measured. For comparison, tests with fossil diesel fuel were performed before and after the endurance test.During the endurance test, the engine was failure-free for 500 hours with the biogenic fuel. There were almost no differences in specific fuel consumption during the test, but the average exhaust gas temperature increased by about 15°C over the time.Emissions changed only slightly during the test. Carbon monoxide, hydrocarbons and aldehydes were effectively eliminated by the oxidation catalyst as part of the PM-Kat®. The concentration of hydrocarbons was partially under background level and the emissions of hydrocarbons as well as carbon monoxide were well under the regulatory requirements. Nitrogen oxides emissions increased, as expected, by 10% when using biodiesel instead of fossil diesel fuel. So they slightly exceeded the legal limit of Euro IV. During the test, small increases of nitrogen oxides, particulate matter and particle numbers were observed.In all, the endurance test demonstrated clearly that the engine can be driven with 100% biodiesel and the biogenic fuel has no effect on the continuously operating PM-Kat®. However, the exhaust gas system might have some optimization potential for biodiesel.