Today in developing countries, carburetors are mainly used in small engines for motorcycle application. Carburetor takes more time for the mixture preparation process, hence the response of the vehicle is sluggish for the rider. To overcome this problem is fuel injection system. The purpose of this work is to study the transient response of a gasoline fuel injection motorcycle engine with different injection parameters. The analysis of the flow is conducted using the commercial CFD software. The simulated results are also compared with the stand start acceleration test results done on the motorcycle, which shows 11% improvement than carbureted vehicle.