We propose an on-glass antenna with vertical lines for FM radio reception in a commercial sedan. The proposed antenna consists of vertical lines and multiple horizontal lines that are also used as defroster lines. The proposed antenna structure is optimized with the GA algorithm in conjunction with the FEKO EM simulator. The optimized antenna was built and installed on an Azera test vehicle (Korean model: TG Grandure 270) from Hyndai-Kia Motors and antenna performances such as reflection coefficients, and bore-sight gain are measured in an anechoic chamber. The optimized antenna shows a half power matching bandwidth of 26 % at the center frequency of the FM radio band and an average bore-sight gain of about −10.37 dBi. Then, we take a field test to measure actual received power of FM radio signals. The field test result shows the antenna's minimum received power level of about −80 dBm. The overall measured data show that the optimized antenna can be used as a FM antenna for vehicles.