The mechanical behavior of a commercially available single layer polypropylene (PP) separator was investigated using a dynamic mechanical analyzer (DMA). Samples were tested along the machine direction (MD) and transverse direction (TD). The tensile stress-strain, tensile creep and viscoelastic behaviors were studied. Experiments were performed in two conditions: (1) dry and (2) wet, i.e., samples submersed in dimethyl carbonate (DMC). The experimental results revealed that the mechanical properties of the separator were much lower while being submersed in DMC. This finding suggests that the mechanical properties measured at a dry condition may not be sufficient to represent the in-situ material behavior. Therefore, it is important to conduct material characterization in an environment close to the service condition.