Fuel sloshing noise is involved with flow motion inside fuel tanks as well as structural characteristics of vehicles. Therefore it is necessary to introduce Fluid-Structure Interaction (FSI) analysis to predict sloshing noise phenomena more accurately. Purposes of this paper are to verify the reliability of the FSI method and suggest new CAE analysis processes to predict fuel sloshing noise. The vibration of floor panels induced by sloshing impact is evaluated through FSI analysis. A series of tests is carried out to validate simulation results. The numerical optimization of parameters is also carried out to reduce computation time. In addition, effects of sloshing noise factors are discussed based on simulation and test results. Lastly, a method to predict fuel sloshing noise by exerting sloshing load on a vehicle is suggested.