Large eddy simulation (LES) of two-phase non-reacting flow in an internal combustion (IC) engine has been carried out. The impact of cyclic velocity variations, unsteady effects of fuel spray injection as well as their joint effect on in-cylinder flow field pattern were investigated. Influence of these factors on globally averaged charge motion was discussed in details. LES of 40 consecutive engine cycles have been performed using KIVA-3V for each considered case. The configuration under investigation represents a four stroke direct fuel injection engine with variable tumble system.