This study derives regression equations for predicting the cetane number of biodiesel fuels based on chemical analysis data. For conducting the regression analysis, 34 fuel samples with a wide variety of ignition qualities were made by mixing five kinds of biodiesels and five kinds of fatty acid methyl ester (FAME) reagents. The relationship between the cetane number, measured in a constant-volume combustion chamber, and fuel properties such as iodine value, saponification number, and boiling point, was investigated. Based on the results, four regression equations were proposed and their accuracies were compared. The results show that the regression equation based on fuel composition gives a cetane number with high accuracy, whereas it can be only be approximately predicted from the iodine value.