The influence of fuel composition on sprays was studied in an injection chamber at DISI conditions with late injection timing. Fuels with high, mid and low volatility (n-hexane, n-heptane, n-decane) and a 3-component mixture with similar fuel properties like gasoline were investigated. The injection conditions were chosen to model suppressed or rapid evaporation. Mie scattering imaging and phase Doppler anemometry were used to investigate the liquid spray structure. A spray model was set up applying the CFD-Code OpenFOAM. The atomization was found to be different for n-decane that showed a smaller average droplet size due to viscosity dependence of injected mass. And for evaporating conditions, a stratification of the vapor components in the 3-component fuel spray was observed.