In this study, an attempt has been made to estimate trapping efficiency of a two-stroke engine by CFD analysis under cold flow conditions. A single-cylinder, loop-scavenged, spark-ignition, two-stroke engine extensively used for two-wheeler application in India is being considered for the analysis. Engine geometry is modeled using commercial PRO-E software. Simulation is carried out using commercial CFD code STAR-CD. The CFD predictions are validated by available experimental data. In the present study, the trapping efficiency is estimated at various engine speeds with change in configurations of ports. From the analysis of results, it is found that, increasing exhaust port area relative to total transfer port area and engine speeds increase the trapping efficiency significantly. In general, it is possible to increase the trapping efficiency by about 4% at the engine speeds considered.