Recent research has focused on engine combustion technology as well as application of after-treatment in order to comply with emission regulation. However, it is much more efficient way to control emissions from engine itself and furthermore research on engine control will provide the direction of after-treatment technology in future. Furthermore, emission standard regulation for passenger diesel vehicles has been stringent compared to others and nano-particles will be included in EURO6 regulation in Europe and similar emission standard will be introduced in Korea.A 3.0-liter high-speed diesel engine equipped with by CRDI system of 160 MPa injection pressure, and an intake/exhaust system of V type 6-cylinder turbo-intercooler was applied. The conditions of fuel spray and combustion were investigated by setting up an ETAS system which is a similar condition to the engine dynamometer test. The engine drives from an engine speed of 2000 rpm to 3000 rpm to provide 160 MPa high pressure injection by using a piezo injector. The injection period and injection quantity, pilot injection types which are related to CRDI and air/fuel ratio control applied by EVGT were changed simultaneously. In order to select optimum injection timing, low engine load and injection quantity were fixed and this experiment was repeated by adjusting an injection timing driven at maximum high speeds. And then air excess ratio, cycle change, indicated pressure diagram, heat release rate and emissions were investigated after injection timing was selected by MBT.Standard experiment procedure constituted dilution apparatus and CPC system to collect nano-particles and these test results were compared with regulated materials of CO, HC, NOx and investigated their relation and characteristics of nano-particles.