Adverse health effects of particulate matter (PM) originating from diesel engine exhaust are largely attributed to the complex chemical composition of the exhaust species. This study was set out to characterize particulate emissions from a Euro-III-compliant modern automotive common rail direct injection (CRDI) sports utility vehicle (SUV) diesel engine operated at different loads at rated engine speed (1800 rpm), employing diesel and 20% biodiesel blends (B20) produced from Karanja oil. This study is mainly divided into two main sections, first one includes the gravimetric analysis in order to assess the amount of Benzene Soluble Organic Fraction (BSOF) and trace metals using Inductively Coupled Plasma-Optical Emission Spectrometer (ICPOES). The second section includes real-time measurements for Organic Carbon (OC), Elemental Carbon (EC) and total particle-bound Polycyclic Aromatic Hydrocarbons (PAHs). For diesel and biodiesel fuels, BSOF results showed decreasing trends with increasing engine load. B20 showed higher BSOF as compared to diesel for all loads. The concentration of the different trace metals analyzed also showed decreasing trends with increasing engine load. OC/EC data suggested that the ratio of OC to EC decreased with corresponding increase in engine load for both fuels. A peak in PAH concentration was observed at 60% rated engine load at 1800 rpm and was almost identical for both fuels. Comparison of chemical components of PM emitted from this CRDI engine provides new insights in terms of PM toxicity for B20 vis-à-vis diesel.