Research studies show that 2-methylfuran (MF) is a promising gasoline alternative regarding its positive effect on engine performance and emissions. Before using pure MF in spark ignition engines, it is more likely to be used in a low-level blended form in gasoline.An experimental research study was carried out to investigate the impacts of low-level MF content in gasoline (volumetric 10% MF in blend) on direct-injection spark-ignition (DISI) engine combustion behavior and emissions. The tests were conducted on a single-cylinder spray-guided DISI research engine at an engine speed of 1500 rpm under stoichiometric conditions. The engine loads of 3.5 ~ 8.5 bar IMEP were tested and gasoline-optimized spark timing was used. Furthermore, the effects of spark timing, exhaust gas recirculation (EGR) and valve overlap on NOx emissions were tested. The results showed that by adding 10% MF to gasoline, the combustion behavior was not changed significantly however the combustion stability has been improved. The HC emissions were reduced and the NOx emissions were comparable. At low engine load (3.5 bar IMEP), retarded spark timing, EGR and increased valve timing all tended to reduce NOx emissions. However adding EGR or retarding the spark timing resulted in reduced indicated efficiency. The increased valve overlap is an ideal strategy to reduce NOx emissions for its less negative effect on combustion stability and improved indicated efficiency, compared to the effect of retarded spark timing and EGR.