The two-load method is commonly used to determine the transmission loss of a muffler or silencer. Several practical measurement considerations are examined in this paper. First of all, conical adapters are sometimes used to transition between impedance tubes and the muffler. It is demonstrated that the effect of adding the adapter can be quite significant at low frequencies especially if the adapter is short in length. The effect of changing the length of the adapter was examined via measurement and plane wave theory. Secondly, the effect of selecting the reference microphone was examined experimentally. It was found that measurements are improved by selecting a downstream reference. Finally, the effect of using different frequency response function estimation algorithms (H1, H2 and Hv) was compared sans flow. This had little effect on the measurement.