The Vehicle NVH performance is the main factor influencing the vehicle ride comfort. With regard to the NVH performance, the interior booming noise problem in run-up condition caused by powertrain vibration is the most serious NVH problem. Lots of researches have been done upon the engine theory exciting force to do the simulation analysis and optimization of the interior vibration and noise performance.In this paper, the vibration excitation of the powertrain mount is measured by testing the mule car and the interior noise are simulated by using the full vehicle Finite Element Analysis (FEA) model. Then the panel contribution analysis (PCA) is implemented on the peak frequency of the results, and the greater influential panels to the interior noise are identified, then the structural modifications and the damping layout optimizations to reduce the interior structure-borne noise are proposed by the analysis. According to the optimization process, the vehicle interior noise performance is improved and meanwhile the effectiveness of this method is also verified.