Owing to the presence of oxygen atoms in biodiesel, the use of this fuel in compression ignition (CI) engines has the advantage of reducing engine-out harmful emissions. In this context, biodiesel fuel can also be used to extend the low temperature combustion (LTC) regime because it inherently suppresses soot formation within the combustion chamber. Therefore, in this study, LTC characteristics of biodiesel were investigated in a single cylinder CI engine; the engine performance and emission characteristics with biodiesel and conventional petro-diesel fuels were evaluated and compared. A modulated kinetics (MK)-like approach was employed to realize LTC operation. The engine test results showed that LTC operation was achieved by retardation of the fuel injection timing. The results also showed that using biodiesel reduced smoke, THC, and CO emissions but increased NOx emissions. The effects of the boost pressure and O2 fraction on engine behavior in both conventional and LTC combustion regimes were examined, and the corresponding emission results are presented and discussed.