This paper describes a prediction of vibration and the transfer path analysis (TPA) using an engine multi body dynamics (MBD) model and measured frequency response functions (FRFs). TPA is used in order to analyze each contribution of vibration transfer paths. In the TPA, input forces from vibration source to passive part should be identified accurately. In the traditional TPA, an identification of input forces is done using only experimental results. Therefore, a parametric study to an improvement of a structure or an isolation system is impossible. In this study, the MBD model of engine is constructed, and input forces from engine to mainframe of agriculture machine are predicted. The accuracy of prediction is confirmed, compared with the results from the traditional TPA method. The contribution of each transfer path is analyzed, and the vibration levels of operator position are predicted using the measured FRFs and the simulated input forces. As a result, the input forces and the vibration levels of operator position can be predicted accurately.