Emission reduction and efficiency increase of two-stroke marine diesel engine depends on fuel injection, which affects in-cylinder combustion. This paper presents injection strategies varied results from simulation based on AVL BOOST and the experimental results and its validity was confirmed by actual measurements in a two-stroke engine during shop testing, which reveals the affect on performance and emission, as well as the corresponding concept analysis. This simulation enabled a more thorough investigation into engine performance features. In particular it provided a detailed examination of how changes in injection strategies impacted the combustion and ultimately engine performance. These results are summarized and based on these results, optimal injection strategies settings are suggested for balancing engine performance and NOx emissions.