As for automobile, the mass production period of Electric Vehicle(EV) has begun by the rapid progress of the battery performance. But for EV- Motorcycle(MC), it is limited for the venture companies' releases.The design and evaluation methodologies are not yet established or standardized so far. This paper provides the practical and the experimental examples. To study the feasibility of EV-MC, we developed the prototypes in the present technical and suppliers' parts environments, and evaluated them by the practical view of the MC usage. The developed EV-MC has the equivalent driving performance of the 250cc internal combustion engine(ICE)-MC and a cruising range of 100km in normal use.In the prototype development, the reliability and the ability of protection design of the battery in the whole vehicle against the environmental loads are mainly studied, especially, heat and cold, water, shock, and the accident impact. In addition, it is carried out the performance improvement by the heat management design of the motor to meet the practical use condition. From the points of the fun to ride factor, we developed the function of the 4-speeds dog gear Manual Transmission(MT) and its electric control, reward ride function, the regenerative brake control, and the quick charge. And by the consideration of maneuverability and the maintenance-ability of the battery in stock, the vehicle chassis is designed.