High silicon molybdenum (HiSiMo) ductile cast iron (DCI) is commonly used for high temperature engine components, such as exhaust manifolds, which are also subjected to severe thermal cycles during vehicle operation. It is imperative to understand the thermomechanical fatigue (TMF) behavior of HiSiMo DCI to accurately predict the durability of high temperature engine components. In this paper, the effect of the minimum temperature of a TMF cycle on TMF life and failure behavior is investigated. Tensile and low cycle fatigue data are first presented for temperatures up to 800°C. Next, TMF data are presented for maximum temperatures of 800°C and minimum cycle temperatures ranging from 300 to 600°C. The data show that decreasing the minimum temperature has a detrimental effect on TMF life. The Smith-Watson-Topper parameter applied at the maximum temperature of the TMF cycle is found to correlate well with out-of-phase (OP) TMF life for all tested minimum temperatures. Fractography and energy dispersive spectroscopy (EDS) are then performed on the tested specimens to determine the difference in failure mechanism as the minimum cycle temperature changes. Based on these observations, it appears that the segregation of magnesium to the grain boundary which occurs near 400°C in HiSiMo DCI influences TMF failure behavior when the TMF temperature cycle passes through 400°C.