The depletion of resources, increased cost of fossil fuel and increased environmental awareness reaching the critical condition. Development of viable alternative fuels from renewable resources is gaining the international attention and acceptance. The vegetable oils have the potential of alternative fuel for compression ignition engines by converting it into biodiesel. The mangifera indica oil is a nonedible vegetable oil, available in large quantities in mango cultivating countries including India. Very little research has been done on utilization of oil in general and optimization of transesterification process for biodiesel production. In the present study, transesterification process with use of homogeneous catalyst has been optimized. Various input parameters like oil-tomethanol molar ratio (1:08, 1:12 and 1:16), catalyst type (NaOH, KOH and NaOCH3), catalyst concentration (0.5, 1 and 1.5 wt %) and reaction temperature (59, 64 and 69°C) were studied by applying the orthogonal experimental array L9.ANOVA (F-test at P=0.05 contribution of each signal to noise factor) technique was used for optimization with the objective of maximizing the yield of high quality mangifera indica oil biodiesel.The optimum conditions for transesterification process are: 1:12oil-to-methanol molar ratio, 1.0 wt.% catalyst concentration, KOH catalyst, &64°C reaction temperature. The optimum yield of MOME was 89.8%. The biodiesel produced (MOME) is within the limits prescribed by EN-14214 standard. The density, flash point and cloud and pour points for MOME were higher than mineral diesel. Comparatively, the higher flash point of MOME makes it a safer fuel to handle. The calorific value of MOME was slightly lower than that of diesel. All these tests for the characterization of MOME demonstrated that almost all properties of MOME are comparable to those of diesel, and this makes it a potential substitute for diesel fuel in compression ignition engines.