The mangifera indica oil is a nonedible vegetable oil, which is available in large quantities in mango cultivating countries including India. Very little research has been done on utilization of oil in general and optimization of transesterification process for biodiesel production. In present study, the transesterification processes with heterogeneous catalyst. The various input parameters like methanol to oil molar ratio (1:08, 1:12 and 1:16), heterogeneous catalyst types (ZnO, MgO and CaO), catalyst concentration (0.5, 1 and 1.5 wt %) and reaction temperature (59, 64 and 69°C) were studied by applying the orthogonal experimental array L9.ANOVA (F-test at P=0.05 contribution of each signal to noise factor) technique was used for optimization with the objective of maximizing the yield of high quality mangifera indica oil biodiesel. The optimum conditions for transesterification process are1:16 methanol to oil molar ratio of, 1.0 wt.% catalyst concentration, CaO as the catalyst type, 69°C reaction temperature. The optimum yield of MOME was 94.6%. The biodiesel produced (MOME) is within the limits prescribed by EN-14214 standard. The density, flash point and cloud and pour points for MOME were higher than those of the mineral diesel. Comparatively, the higher flash point of MOME makes it a safer fuel to handle. The calorific value of MOME was slightly lower than that of diesel. All these tests for the characterization of MOME demonstrated that almost all characteristics of MOME are comparable to those of diesel, and this makes it a potential substitute for diesel fuel in compression ignition engines.