The influence of the eddy scale of initial turbulence in RCM on the pressure rise rate after spontaneous ignition of end gas was investigated. The combustion time of the end gas after spontaneous ignition was observed by using high-speed direct photography. As a result, the large scale eddy reduced the pressure rise rate after spontaneous ignition. The temperature inhomogeneity of end gas was higher with the large scale eddy. The combustion time of end gas after spontaneous ignition was prolonged by variation in local ignition delay due to inhomogeneity. The large scale eddy may prevented the knocking occurrence.