Fuel injection and fuel-air mixture formation processes have significant influence on the performance of spark ignition gas engines. In order to study the fuel enrichment injection process in the pre-chamber of a marine gas engine, the flow field in the pre-chamber during the gas fuel injection period was investigated by the particle image velocimetry (PIV) method. An organic glass model of pre-chamber was made for optical measurement. The flow fields in the pre-chamber with four different gas injection angles were analyzed, respectively. The measurement results were qualitatively compared to the CFD calculation results as the verification of the calculation. Based on the comparison of the PIV experiment results, an optimal gas fuel injection angle was chosen. Furthermore, 3D CFD calculation models with the baseline and optimal fuel injection angles of a marine spark ignited natural gas engine were generated to calculate the working process. The simulation results indicate that compared with the baseline engine, the engine with optimized injection angle has improved flame propagation in the main combustion chamber and reduced NO emission.