PFI (Port Fuel Injection) gasoline engines for motorcycles have some problems such as slow transient response because of wall wet of fuel caused by the injector's layout. Hence, it is important to understand the characteristics of fuel sprays such as droplet size and distribution of fuel concentration. Considering the spray formation in a port, there are three kinds of the essential elements: breakup, evaporation and wall impingement. However, it is difficult to observe three of them at the same time. Therefore, the authors have made research step by step.In the authors' previous study, the authors focused on the wall collision, droplet sizes, droplet speeds and the space distribution of the droplets. In this study, the authors focused on evaporation. A direct sampling method using FID (Flame Ionization Detector) for evaporating fuel was established and the concentration distribution of evaporating fuel in the port was measured and analyzed. As a result, it was found that higher velocity in the port increases fuel concentration with enhanced atomization and that evaporating fuel is easier to be affected by the flow and fuel distillation characteristics.