It is difficult to control the ignition timing of Homogeneous Charge Compression Ignition (HCCI) engines because they lack a physical means of igniting the mixture. Another issue of HCCI engines is their narrow operating range owing to the occurrence of misfiring at low loads and abnormal combustion at high loads. As a possible solution to these issues, this study focused on the generation of a streamer discharge using nonequilibrium plasma as a means of assisting HCCI combustion. A two-stroke engine that allowed visualization of the entire bore area was used in this study. A primary reference fuel blend (50 RON) was used as the test fuel. The streamer discharge was continuously generated in the end-gas region during a 360 deg. interval from the scavenging stroke to the exhaust stroke using a spark plug from which the ground electrode had been removed. Experiments were conducted in which the applied voltage of the streamer discharge was varied to investigate its effect on combustion. The results showed that the application of the streamer discharge to an HCCI engine has the effect of advancing the ignition timing and moderating the rapid rate of combustion. It was also observed that these characteristics can be varied depending on the applied voltage of the streamer discharge.