A comparative study was performed by use of blends of Jatropha oil-diesel fuel and Jatropha oil-kerosene in order to investigate the feasibility of direct utilization of Jatropha oil in a DI diesel engine. Experimental results at low load demonstrated that mixing 60 vol.% of Jatropha oil into both diesel fuel and kerosene gave less impact on indicated thermal efficiency, whereas further increase of Jatropha oil deteriorated it. Jatropha oil-kerosene decreased particulate matter compared to Jatropha oil-diesel fuel, although particulate matter increased with the increase of Jatropha oil fraction. At partial load where double injection was applied, mixing 80 vol.% of Jatropha oil gave no significant impact on indicated thermal efficiency, exhaust gas emissions and particulate matter and no significant difference was observed between diesel fuel blends and kerosene blends. Combustion visualization was also performed in an optically accessible engine in order to gain insight into the ignition and combustion processes at low load.