The purpose of this work is to present an experimental methodology to characterize the in-cylinder tumbling flow generated by a motored four-valve spark ignition single cylinder optical research engine. High-speed two-dimensional particle image velocimetry measurements were made in the symmetry vertical plane between the inlet and outlet valves. The velocity flow fields were recorded during the intake and compression strokes for three different engine speeds (1000, 1500 and 2000 rpm) at several crank angles. Vorticity, tumble ratio and kinetic energy were calculated and compared. Also, an evolution analysis of the main vortex center was made.As result, the tumble ratio and kinetic energy showed a decrease at the end of intake stroke. Flow field cyclic variation could be noticed. The methodology contributes to a better understanding of flow motion behavior, and consequently, the mixture formation process in spark ignition engines.