This paper presents the experimental analysis of separation in vertical headers based on flow visualization. Two-phase separation phenomena inside the header is observed and quantified. Driving forces are analyzed to study the mechanisms for two-phase flow motion and flow regimes. Main tube of the header is made of clear PVC for visualization study. R-134a is used as the fluid of interest and the mass flux from the inlet pass is 55 kg/m2s - 195 kg/m2s. Potential ways to improve two-phase separation are discussed. A model is built to show how separation brings potential benefits to MAC heat exchangers by arranging the flow path.