An experimental study was conducted to evaluate the variable amplitude fatigue behavior of a neat polymer (polypropylene impact co-polymer) and a polymer composite made of polybutylene terephthalate (PBT) with 30 wt% short glass fibers. Fatigue tests were conducted on un-notched and notched specimens at room temperatures. Plate-type specimens were prepared in the transverse direction with respect to the injection mold flow direction and a circular hole was drilled in the center of notched specimens. Two-step loadings (high-low and low-high) tests at two damage ratio of 0.2 and 0.5 at stress ratios of R = 0.1 and -1 were conducted to investigate load sequence effects and prediction accuracy of the linear damage rule. Different behaviors were observed for unreinforced and short glass fiber reinforced polymers under the two-step loading tests. Periodic overload tests were also conducted to evaluate the effect of overloads during cyclic loading on fatigue life and this effect was found to be less pronounced for polymeric materials, as compared to metallic materials.