The spray characteristics is the key to achieve the clean combustion in diesel engines and the in-cylinder conditions are one of the factors affecting the spray process. In this work, the diesel spray characteristics were studied over a range of injection pressures and ambient pressures in a constant volume chamber and a single-hole common rail diesel injector was used. The present work is to decouple the effects of ambient pressure and ambient density on near-field spray processes by using different ambient gas (N2, and CO2). The spray processes were captured by a Photron SA X2 camera with speed of 300,000 fps and resolution of 256 by 80 pixels. The spray processes were analyzed in terms of penetration length and spray tip velocity. Difference in penetration length and tip velocity were found at the same ambient density and/or ambient pressure when different ambient gases were used. This may be attributed to the different gas compressibility and the intensity of momentum transfer between liquid and ambient gas.