The injection amount per stage in a multiple injection strategy is smaller than a conventional single-stage injection. In this paper, the effect of the injection amount (0.27mg, 0.89mg, 2.97mg) under 100MPa injection pressure and the effect of injection pressure (100MPa, 150MPa, 170MPa) under different injection amounts (0.27mg, 2.97mg) on the spray and mixture formation characteristics were studied by analyzing the vapor/liquid phase concentration distributions obtained under various conditions via using the tracer LAS technique. The spray was injected into a high-pressure and high-temperature constant volume vessel by using a single-hole nozzle with a diameter 0.133mm. The higher the injection pressure with a smaller injection amount is, the shorter the spray tip penetration and leaner air-fuel mixture occur. The combustion processes had been examined by a high-speed video camera with the two-color pyrometry method. The distributions of KL factor and flame temperature had been investigated with 100MPa injection pressure under different injection amounts. The results show that a small injection amount gives a small flame area and a low soot concentration.