This paper presents the physical adjustments and the incorporation of a control system to injection, ignition and throttle valve parameters for operation of a diesel engine with 100% hydrous ethanol. The control system of the aforementioned parameters integrates three dependent subsystems. The control systems of the throttle valve opening, fuel injection and ignition timings have the purpose to reduce cylinder pressure, control engine speed and the combustion process. The signals generated depend on engine speed and knock sensors, which are operated by microcontrollers programmed in Assembly and C language. The measured parameters during engine operation are relative humidity, temperature in different engine locations, fuel consumption and intake air mass flow rate. The data collected are monitored by a software developed in LabVIEW platform. The software also controls the load applied at each engine operating condition.