Manganese sulfides (MnS) are nonmetallic, ductile inclusions with high melting temperature (1610 °C) which improve the machinability and retard the grain growth in steels, in addition of contributing to avoid cracking during hot working. In this paper, the effect of manganese sulfides on the fatigue life of the vanadium micro-alloyed forging steel 38MnVS6 is discussed. Force-controlled fatigue tests are performed on small sized specimens until the crack occurs. The fatigue life of the forged material, presented by Wöhler curves, is considerably reduced at high levels of the nominal stress amplitude compared to the wrought material. Moreover, it is evident that the presence of longer and thinner particles of MnS reduces the scatter band of Wöhler curves and decreases the fatigue strength of the material. This paper presents a first attempt to find a relation between the shape and content of manganese sulfides due to the forging process and the fatigue life of the material.