The sewing machine has been widely used in various aspects of life and it’s essential to study its kinematic and dynamic characteristics. A dynamic model of flexible multi-link mechanism for sewing machine including joints with clearance is established to analysis its dynamic response in the present work. The configuration of the sewing machine included six subsystems, feed mechanism, needle drive mechanism, looper drive mechanism, thread take-up mechanism, knife drive mechanism and lubrication mechanism. Since the sewing machine mainly consists of linkage mechanisms which are connected by revolute joints and translational joints, the existence of clearances in the joints and the flexibility of crank shaft and linkage are important factors that affect the dynamic performance. Even little clearance can lead to vibration and fatigue phenomena, lack of precision or even make overall behavior as random. The dynamic response of the sewing machine with multi-clearances is discussed and compared between the mechanism with multi-rigid components and with a flexible crank shaft and linkages. It is demonstrated that the dynamic response of the mechanism is greatly influenced by the clearance and agrees better with realistic situation when taking the effects of flexibility into consideration. The influence of the clearance size, input crank shaft speed, and number of clearance joints on the dynamic response of the mechanism is also investigated.