Rubber gaskets, for example O-rings, and rubber valves achieve their sealing performances when compressed rubber material fills in the gap between the gasket and the counter surface and a continuous contact is formed between them. Leaking is likely to occur at low temperature since it is difficult for the rubber gasket to deform along the roughness of counter surface because of decreasing in rubber elasticity. However, sticking to the counter surface prevents the gasket from ease removing from it due to sealing pressure and sealing fluid from leaking through the gap. It is considered that sticking affects sealing property of rubber gaskets at low temperature. Therefore we studied effects of rubber material, roughness of counter surface and temperature sequence on sealing property by measuring sticking force and observing contact area. The sealing property depended on sticking force at low temperature and enhancement of sticking force enabled to seal at higher pressure. The sticking force was influenced by compression ratio, roughness of counter surface, temperature sequence and contact time. It was suggested that sticking force was caused by physical interaction because rubber component hardly transferred to counter surface after the contact.