The interaction of fuel sprays and in-cylinder flow in direct-injection engines is expected to alter kinetic energy and integral length scales at least during portions of the engine cycle. High-speed Particle Image Velocimetry (PIV) was used in an optical four-valve, pent-roof spark-ignition direct-injection (SIDI) single-cylinder engine to quantify this effect. Two-dimensional velocity fields were measured with crank angle resolved time steps and a field-of-view to enable the determination of integral length scales through critical portions of the engine cycle. Non-firing motored engine tests were performed at 1300 RPM with and without fuel injection to quantify the impact that the fuel sprays have on the in-cylinder flow. The fluctuations of the engine flow were quantified with fuel injected in early intake and late compression strokes, representative of quasi-homogenous and stratified combustion strategies. The evolution of the average and turbulent kinetic energy shows the significant impact that both early and late injection strategies have on in-cylinder flow. Furthermore, the data shows that spray-flow interactions substantially affect the integral length scale throughout the cycle and also produces strong spatial variations by a factor of 5.