This article presents the effects of indicated mean effective pressure (IMEP), spark timing, compression ratio (CR), and exhaust gas recirculation (EGR) on the cyclic variation and individual cycle characteristics a of spark ignition engine fueled with gasoline, 2-methylfuran (MF) blends with various MF volumetric fractions from 0% to 100%. In addition, the coefficient of variation of the indicated mean effective pressure (COVIMEP) is used to quantify the effect of varying engine parameters on the combustion variation with gasoline-MF blends. The test results show consistently that the COVIMEP of gasoline-MF blends decreased (with increasing MF volume fraction) compared with that of gasoline. More specifically, when the spark advance increases, the COVIMEP first decreases and then increases for all the test fuels, and reached the lowest COVIMEP at maximum brake torque (MBT) spark advance for each of the test fuels. It was found that it was beneficial to the COVIMEP to increase IMEP and CR for all fuels, especially gasoline. Compared with the IMEP, the CR showed less effect on the cycle variation. Unfortunately, EGR had a negative impact on cyclic variation, addition of MF in gasoline results in lower COVIMEP when higher EGR rate was used.