Hydrogen internal combustion engine has advantages in many aspects compared with traditional internal combustion engine. E.g. hydrogen is renewable energy and its source is more widely and more easily, so it can alleviate the traditional energy crisis; the hydrogen internal combustion engine has the higher efficiency and the lower emissions. The structure of hydrogen internal combustion engine is highly consistent with the traditional internal combustion engine, so users do not need to change a lot when using hydrogen internal combustion engine. The combustion characteristics of hydrogen-air mixture are great significance for the performance and control of hydrogen internal combustion engine and it is also the theory and technical difficulties in the process of designing hydrogen internal combustion engine. The model that can accurately predict the mixture combustion velocity in cylinder is very important. The combustion velocity is an important parameter for mixture combustion. Taking a four-cylinder hydrogen engine as the research object, combined with the constant volume bomb to test mixture combustion and made the calculation and analysis of hydrogen turbulent premixed combustion. Hydrogen turbulent combustion belongs to fold laminar flame model. The turbulent combustion velocity is related to not only turbulent and laminar combustion velocity but the turbulent add-ons effects of flame instability when the hydrogen combustion is lean. Put forward the calculation formula which is considering the instability of turbulent combustion and test validation. The formal can be used to calculate the combustion velocity of hydrogen internal combustion engines and it is useful to the design and optimization of hydrogen internal combustion engine.