One of the most promising platforms for cellulosic biofuel generation is to harness the metabolic processes of endophytic fungi that directly convert lignocellulosic material into a variety of volatile organic compounds. The biofuel co-development framework was initiated at Sandia National Labs. Here, the synthetic biologists develop and engineer a new platform for drop-in fuel production from lignocellulosic biomass, using several endophytic fungi including Hypoxylon CI-4A, CO27-A, and Daldinia EC-12. Hence this process has the potential advantage that expensive pretreatment and fuel refining stages can be optimized thereby allowing scalability and cost reduction-two major considerations for widespread biofuel utilization. Large concentrations of ketones along with other volatile organic compounds (VOC’s) were produced by Hypoxylon CO27-A grown over swtichgrass media. The combustion and emission properties of these novel fungal biofuels (large ketones) are poorly known, and fundamental measurements of representative molecules are needed in order to provide feedback on their desirability in advanced combustion engines (e.g., HCCI: homogeneous, charge, compression and ignition engines) and their impact on emissions as well as other combustion devices such as micro combustors. The 2,4-Dimethyl-3-pentanone (DIPK) is a promising candidate biofuel for automotive applications produced by the fungal conversion process. Laminar burning velocity is an important fundamental property of a fuel/air mixture and it depends on the composition, temperature, and pressure. Therefore, the knowledge of the dependence of the laminar burning velocity on above mentioned parameters can be used to design advanced ICE’s as it can affect efficiency and heat release rates. We provide measurements of laminar burning velocities in UCF’s spherical flame chamber using high-speed visualization. Flame radius as a function of time and burning velocity data were obtained from post-processing and numerical analysis of the Schlieren images. In addition, direct visualization was utilized to obtain information about flame stretch, which is important in turbulent flames.