The interaction of three bio-lubricant base oil candidates with seventeen combinations of surface treatment were studied, comparing wear scar volumes and coefficient of friction results. Substrates were initially ground or superfinished, a combination of Dymon-iC™ DLC, an ultra-fine shot blasting method doped with Tin and Molybdenum Disulfide, a calcium based chemical dip and nano fullerene, were used. DLC is a well reported to reduce frication and wear, some reports suggest wear in coated contacts is independent of the type of lubricant used, others report that bio-lubricants offer reduced friction and wear in combination with DLC. Shot blasting can also reduce wear and friction, dimples act as lubricant reservoirs, making hydrodynamic lubrication more likely. Work has also explored the performance of surface texturing in combination with coatings, some finding higher friction with surface texturing and DLC. As a surface coating, fullerene has been shown to have significantly lower wear and friction then DLC coatings. The calcium based chemical treatment used has no published data. A ball on flat reciprocating wear tester was used with bio-lubricant base oil candidates, jojoba, soybean and palm oil, with a mineral base oil used for comparison. Wear scars were analysed using a Scan Electron Microscope and a Profilometer. Tests performed with bio-base oil candidate jojoba oil gave coefficient of friction results lower than the other bio-base oils tested, while the mineral base oil gave the highest coefficient of friction results. The chemical dip surface treatment gave comparable wear protection to DLC. Results showed little difference when substrates were shot blasted before the DLC coating was applied. When shot blasting was combined with the chemical dip there was a noticeable reduction in the size of wear scars. Superfinishing substrates improved the performance of both the chemical dip and DLC, but not with shot blasting alone.