The transmission spinloss has significant influences on the vehicle fuel economy. Transmission output chain may have 10~15% contribution to it. However, the chain spinloss information is very rare. It is challenge to give a good estimation of the chain spinloss. An experimental study was carried out with several transmission output chains and simulated transmission environment in a testing box. The studies build the bases for the chain spinloss modeling and depicted the influences of the speed, the sprocket sizes, the oil levels, the viscosity, the temperatures and the baffle. The kriging was employed for the parameter sensitivity study. The results indicate that the speed, the sprocket width, the oil height and the viscosity are the top influencers depending upon the speed range.