In addition to the analysis of human driving behavior or the development of new advanced driver assistance systems, the high simulation quality of today’s driving simulators enables investigations of selected topics pertaining to driving dynamics. With high reproducibility and fast variant generation subjective evaluation process leads to a better system understanding in the early development stages. With the opportunity of a realistic whole-vehicle simulation provided by the Stuttgart Driving Simulator fundamental analysis of the human’s thresholds of perception are carried out. The thresholds give general information about the perception of characteristic stimuli pertinent to driving dynamics, which are caused by road unevenness or bumps and transferred over the vehicle body to the occupants. Furthermore, objective data are generated to support the correlation of subjective evaluation and objective data. A detailed five-mass vehicle model, synthesized road profiles and measured road excitations provide the basis of this analysis to obtain more realistic results. The transfer of the original on-road test run to the virtual reality of the driving simulator includes the full flexibility of the vehicle model and the design freedom of the scenario, which allows new possibilities of investigation. Based on real test runs on the autobahn the vehicle’s body motion is measured after overrunning the road unevenness and exactly implemented in the driving simulator. For more detailed evaluation the excitation is divided into single impulses according to the vehicle’s degrees of freedom. In the present case yawing and rolling motion are considered separately. The driver can modify amplitude and phase of the single impulses by control elements to get an immediate subjective impression of the resulting vehicle motion. With this method new objective criteria can be derived to enhance the basic design of driving dynamics.