Purpose: For rear-wheel-drive (or all-wheel-drive) vehicles, the vibration and noise that caused by driveshaft often become the main factors that influence the comfort level of vehicle. In order to control and improve the NVH problems related to driveshaft, this work aimed to study the excitation mechanism and transfer path of driveshaft vibration, and then to propose effective measures. The purpose of this work was to propose an effective way to improve the NVH performance by controlling the additional excitation force of U-joints in the early phase of project. Methodology: First, the rotation order characteristics of driveshaft were studied with the aid of classical dynamics. Then a rigid-elastic coupling model of vehicle powertrain was modelled with the theory of multi-body dynamics. By inputting the actual vehicle parameters into the model, the acceleration operation of vehicle could be simulated. The simulated dynamical results of concerned component would indicate the vibration characteristics and the transfer path that related to the exaction of rotating driveshaft. After verifying the feasibility of this simulation method via direct measurements, the NVH performance of the built vehicle model was improved through sensitivity analysis and parameter optimization. Results: Both of the classical dynamics calculation and MBS simulation indicated that, the second-order exacting force of driveshaft relates to the angles between each two pieces of shaft and the angles between the yokes of the cross U-joints. The second order NVH problems could be improved by decreasing the angles mentioned above or by optimizing the isolation characteristics of mounting system and driveshaft supports.