In recent times, due to the improvement of internal cylinder flow analysis technology with Computational Fluid Dynamics (CFD), the prediction accuracy of fuel consumption and emission has improved. However, small motorcycles often have complex intake ports which restrict the layout of injectors. Therefore optimization of injection spray to achieve high combustion efficiency and less wall wetting is a challenge. In this study, we predicted fuel consumption and emission performance by the simulation result of air fuel distribution and wall wetting amount with an actual motorcycle engine model. We optimized injector nozzle length, spray angle and spray tip penetration. After the optimization, we evaluated the emission performance and fuel consumption with an actual engine. As a result, we were able to confirm the improvement of fuel consumption and emission performance.