Small gasoline engines are used in motorcycles and handheld machinery, because of their high power density, low cost and compact design. The reduction of hydrocarbon emissions and fuel consumption is an important factor regarding the upcoming emission standards and operational expenses. The scavenging process of the two-stroke engine causes scavenging losses . A reduction in hydrocarbon emissions due to scavenging losses can be achieved through a better understanding of the inner mixture formation. The time frame for fuel vaporization is limited using two-stroke SI engines by the high number of revolutions. With crank angle resolved optical methods it is possible to analyze the mixture formation behavior and combustion. A topic of these investigations is the use of alternative fuels such as alcohol- or butanol-blends and the analysis of their impact on the engine behavior. These fuels show high potential for further reduction of exhaust emissions while simultaneously increasing the engine power output.