Non-reacting spray behaviors of the Engine Combustion Network “Spray G” gasoline fuel injector were investigated at flash and non-flash boiling conditions in an optically accessible single cylinder engine and a constant volume spray chamber. High speed mie-scattering was used to determine transient liquid-phase spray penetration distances and observe general spray behaviors. The standardized “G2” and “G3” test conditions recommended by the Engine Combustion Network were matched in this work and the fuel was pure iso-octane. Results from the constant volume chamber represented the zero engine speed condition and single cylinder engine speeds ranged from 300 to 2000 RPM. The results of the present work clearly illustrate the significant effects of in-cylinder airflow on general fuel spray behaviors and support the notion that behaviors in a constant volume spray chamber are not wholly representative of those in a real engine. Overall, increasing engine speed correlated with enhanced spray breakup and spray plume interactions, leading to the formation of a unified but highly irregular plume in the engine not seen in the spray chamber. The central location of this irregular plume moved away from the injector tip as engine speed was increased as well, further illustrating the confounding of charge motion and spray dynamics. With respect to penetration for both flash and non-flash boiling conditions, as engine speed increased the steady-state liquid-phase penetration distance both decreased and was achieved more quickly. This observation is evidence of enhanced vaporization likely resulting from higher levels of turbulence expected with higher piston velocities. The present work represents the first evaluation of the “Spray G” gasoline injector in an engine and provides important baseline data from which more realistic and valuable spray models can be developed.